ImageJ Resources

http://rsbweb.nih.gov/ij/
http://www.fmhs.auckland.ac.nz/sms/biru/facilities/analysis_resources.aspx

Learning ImageJ

ImageJ is a fantastic light-weight tool to analyze and manipulate images. I needed to use it for quickly analyzing microscopy images. Here I will list features of ImageJ that I found particularly useful for my purposes:
- I took a bunch of images at various gains to find out the point where bleaching starts occurring. So I have a series of images in which the pixel intensity varies from 0 to 255.

ImageJ allows me to load the images as a stack and calculate the maximum pixel value in each image by selecting the whole image and hitting 'm' (for measure). Plotting the maximum value gives this:

So, bleaching occurs somewhere between a gain of 450 and 475.