ImageJ is a fantastic light-weight tool to analyze and manipulate images. I needed to use it for quickly analyzing microscopy images. Here I will list features of ImageJ that I found particularly useful for my purposes:
- I took a bunch of images at various gains to find out the point where bleaching starts occurring. So I have a series of images in which the pixel intensity varies from 0 to 255.
ImageJ allows me to load the images as a stack and calculate the maximum pixel value in each image by selecting the whole image and hitting 'm' (for measure). Plotting the maximum value gives this:
So, bleaching occurs somewhere between a gain of 450 and 475.
- I took a bunch of images at various gains to find out the point where bleaching starts occurring. So I have a series of images in which the pixel intensity varies from 0 to 255.
ImageJ allows me to load the images as a stack and calculate the maximum pixel value in each image by selecting the whole image and hitting 'm' (for measure). Plotting the maximum value gives this:
So, bleaching occurs somewhere between a gain of 450 and 475.
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